amino acids 1 829  (Addgene inc)

Journal: European Journal of Biochemistry

Article Title: Comparison of expression patterns and cell adhesion properties of the mouse biliary glycoproteins Bgp1 and Bgp2

doi: 10.1046/j.1432-1327.1999.00660.x

Figure Lengend Snippet: Structure of the Bgp1 and Bgp2 genes and transcripts . The structure of the mouse Bgp1 and Bgp2 genes are highly similar in the intron/exon topology and in the length of the introns. Dashed lines represent the alternative splicing patterns giving rise to the respective Bgp1 and Bgp2 transcripts drawn under each gene. The translational start codon (ATG) as well as the stop codon specific for each transcript [TGA (S) or TGA (L)] are indicated. The 5′ and 3′ untranslated regions (5′UT and 3′UT) are included in the open boxes. L, Leader sequence; N, N‐terminal domain; A1, B, A2 encode the C2‐set Ig‐like domains; TM, transmembrane domain; Cyt, the cytoplasmic domain. The arrows under the Bgp2 gene or transcripts are the oligonucleotides used in the Southern‐blot hybridizations in Fig. 2 . The nomenclature for the Bgp transcripts is the following: the name of the gene ( Bgp1 or Bgp2 )/the number of extracellular Ig‐like domains followed by the length of the cytoplasmic tail. sBgp2, baculovirus‐expressed His 6 ‐tagged secreted Bgp2.

Article Snippet: A Bgp2 cDNA fragment including nucleotides 1–829, corresponding to amino acids 1–243 in the extracellular domains of the Bgp2 protein, was cloned into pAcMP2 (PharMingen, San Diego, CA, USA).

Techniques: Sequencing, Southern Blot